Hiveg WL (Wallerstein Laboratory) media are formulated as described by Green and Gray for the examination of materials encountered in brewing and for industrial fermentations containing mixed flora of yeast and bacteria. WL Nutrient and WL Differential Media are used in combination. The WL Nutrient Agar plate is incubated aerobically to give a total yeast count while one WL Differential Agar plate gives the count of acetic acid bacteria, Flavobacterium, Proteus and thermophilic bacterial count when incubated aerobically. The other WL Differential Agar Plate is incubated anaerobically for the growth of lactic acid bacteria and Pediococcus.
Application: For Cultivation & Isolation of Microorganisms Encountered in Brewing & Industrial Fermentation Processes
- Appearance of powder: greenish yellow coloured, homogeneous, free flowing powder
- Gelling: firm, comparable with 2.0% agar gel of MV115 or MV1060
- Colour and clarity: bluish green coloured, very slightly opalescent gel forms in petri plates, clear solution in tubes
- Reaction of 8.02 % w/v of MV115 and MV1060 or 6.02% w/v of MV050 and MV410 aqueous solution is pH 5.5 ± 0.2 at 25°C
- Cultural characteristics observed after incubation at 35°C for 48 hours (MV050 & MV115)