Synonym: Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase
CAS Number: 9002-10-2
Enzyme Commission (EC) Number: 1.14.18.1
MDL Number: MFCD00082118
Biochem/physiol Actions
Converts tyrosine to L-DOPA or tyramine to dopamine
Unit Definition
One unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine.
Application
Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. 1 Tyrosinase has also been used in a study to investigate the oculocutaneous albinism phenotype in the Pakistani population.
General Description
Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. This enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper.
Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. This enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper.
Physical properties
Isoelectric point (pI): 4.7-5
pH optimum is 6-7
Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.
Form: lyophilized powder
Featured Industry: Diagnostic Assay Manufacturing
Storage Temp.: −20°C