Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure. CF® dyes are Biotium's line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. • Green fluorescent CF®488A has excitation/emission at 490/515 nm. • Orange fluorescent CF®543 dye has excitation/emission at 541/560 nm. • Red fluorescent CF®568 dye has excitation/emission at 562/583 nm. • Deep-red fluorescent CF®594 dye has excitation/emission at 593/614 nm. • Far-red fluorescent CF®640R dye has excitation/emission at 642/662 nm. • Far-red/near-IR fluorescent CF®680R dye has excitation/emission at 680/701 nm. Kit Components • CF® dye or biotin-tyramide stock solution • HRP Streptavidin • BSA for blocking • Tyramide Amplification Buffer Plus* • 30% Hydrogen Peroxide*