CAS Number: 11089-65-9
Beilstein Registry Number: 6888090
MDL Number: MFCD00065709
PubChem Substance ID: 24900473
Preparation Note
Tunicamycin dissolves in methanol at 4.9-5.1 mg/ml and yields a clear to very slightly hazy, colorless to yellow solution. It is also soluble in DMSO at 10 mg/ml and yields a clear solution. Furthermore, tunicamycin is soluble in DMF (>10 mg/ml), pyridine (>10 mg/ml), water (<5 mg/ml, pH 9.0), dioxane (<1 mg/ml) and THF. However, it is insoluble in other organic solvents such as acetone, chloroform, and ethyl acetate, and in aqueous solutions with pH <6. Aqueous solutions can be prepared from stock solutions by diluting with water at pH 8-10 or with buffers with pH >7, preferably >8. Tunicamycin will not dissolve in phosphate buffer, pH 8, at 1 mg/ml, even with heating, but solubility can be achieved by raising the pH to 9 and back titrating to pH 7-8.
Biochem/physiol Actions
Antibacterial and antifungal. Blocks the formation of protein N-glycosidic linkages by inhibiting the transfer of N-acetylglucosamine 1-phosphate to dolichol monophosphate. Inhibits bacterial and eukaryote N-acetylglucosamine transferases and prevents formation of N-acetylglucosamine lipid intermediates.
General Description
Contains homologues A,B,C, and D. Composition may vary from lot to lot. Actual content given on label.
Quality: PREMIUM
Solubility: methanol: soluble4.9-5.1 mg/mL, clear to slightly hazy, colorless to yellow, methanol: soluble4.9-5.1 mg/mL, 95% ethanol: soluble1 mg/mL, clear to faintly hazy, THF: soluble <1 mg l, dioxane: soluble <1 mg l, dmf: soluble>10 mg/mL, DMSO: soluble10 mg/mL, clear, pyridine: >10 mg/mL, acetone: insoluble, aqueous base: insoluble, chloroform: insoluble, ethyl acetate: insoluble
Storage Temp.: 2-8°C