Ready-to-use Tryptic (Trypticase) Soy Agar (TSA) Plates with Kanamycin are for growth of nonfastidious and moderately fastidious microorganisms (e.g., Neisseria, Listeria, and Brucella). The combination of soy and casein peptones supply organic nitrogen in the form of amino acids and polypeptides, making the medium highly nutritious. Sodium chloride is added to maintain the osmotic equilibrium.
Kanamycin is used to select for mutated or transformed cells that are kanamycin resistant. In research labs, there are several commonly used kanamycin resistance genes (e.g., aph, ntpII, and aaC-aphD). Typically, low-copy-number plasmids require less kanamycin than high-copy-number plasmids. 50 µg/mL kanamycin is the most commonly used concentration.
Contains 1.5% (15 g/L) tryptone, 0.5% (5 g/L) soytone, 0.5% (5 g/L) sodium chloride, 10 µg/mL or 50 µg/mL kanamycin, and 1.5% agar.