Trypsin from porcine pancreas lyophilized powder, Type II-S, 1,000-2,000 units/mg dry solid

CAS Number: 9002-07-7

EC Number: 232-650-8

Enzyme Commission (EC) Number: 3.4.21.4

MDL Number: MFCD00082094

Analysis Note
One BTEE unit = 320 ATEE units

General description
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding. ⁴

Unit Definition
One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Physical form
Lyophilized powder containing lactose

Application
Trypsin can be used in immunohistochemical procedures to enhance staining and to unmask antigens after routine fixation and processing. Trypsin from porcine pancreas has been used in a study to assess homotropic cooperative binding of organic solvent vapors. 1 Trypsin has also been used in a study to investigate the strong purifying selection against gene conversions in primates. ²

Tyrpsin is a serine protease used to hydrolyze proteins. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins ³. Product 93613 is from porcine pancreas and has a molecular weight of 23.8kDa. Trypsin from porcine pancreas has been used in various in-gel digestions.

Tyrpsin is a serine protease used to hydrolyze proteins. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins ³. Product 93615 is from porcine pancreas and has an activity of about 1500units/mg. Trypsin from porcine pancreas has been used in various in-gel digestions.

Biochem/physiol Actions
Tyrpsin cleaves peptide chains mainly at the carboxyl side of the amino acids lysine or arginine. Tyrpsin plays an important role in digestion and protein absorption. Tyrpsin originates in the pancreas in the form of the zymogen, trypsinogen³.