Purity: Identity and purity qualitatively determined by (SDS-PAGE)
Storage: -20C
EC Number: 232-701-4
UNSPSC Code: 12352200
Features and Benefits: Tropoelastin available in its native state. Unique since it becomes cross-linked immediately after its synthesis by the cell and during its export into the extracellular matrix.
Application: Preparation ProcedureUse this procedure as a guideline to determine optimal coating conditions for the culture system of choice.1. Add 1 ml of sterile 0.25% glacial acetic acid solution to the tropoelastin serum vial containing 1 mg.2. Mix contents gently until material is completely solubilized. The solution may remain slightly hazy.3. Transfer desired volume of solution from the serum vial to a dilution vessel if required. Further dilute to desired concentration using sterile 0.25% Acetic Acid solution. A typical working concentration may range from 1 to 50 mug/ml.4. Add appropriate amount of diluted tropoelastin material to the culture surface.5. Incubate at room temperature or 37 C, covered, for approximately 1.5 hours.6. After incubation, aspirate any remaining material.7. Rinse coated surfaces carefully with sterile water and avoid scratching surfaces.8. Coated surfaces are ready for use. They may also be stored at 2-10 C damp or air dried if sterility is maintained.Note: To maintain sterility, perform all operations in a laminar flow hood employing aseptic techniques.
Other Notes: Storage at -20 C prior to solubilization is recommended. Storage at 2-10 C after reconstitution is recommended.Shelf life is 24 months when stored at -20 C. Shelf life after reconstitution is 3 months when stored at 2-10 C
RIDADR: NONH for all modes of transport
WGK Germany: 3