Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride
CAS Number: 1185-53-1
Linear Formula: NH2C(CH2OH)3 · HCl
Molecular Weight: 157.60
Beilstein Registry Number: 3675235
EC Number: 214-684-5
MDL Number: MFCD00012590
PubChem Substance ID: 24889855
Other Notes
Component of extraction buffer e.g. phenol extraction of DNA or RNA; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE.
Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Grade: for molecular biology
Product Line: BioUltra
Assay: ≥99.0% (AT)
Impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
Ign. Residue: ≤0.2% (as SO4)
Loss: ≤0.2% loss on drying, 110°C
pH: 2.5-4.0 (25 °C, 4 M in H2O)
Useful pH Range: 7.0 - 9.0
pKa (25°C) : 8.1
Solubility: H2O: soluble4 M at 20°C, clear, colorless
Anion Traces: sulfate (SO42-): ≤50 mg/kg
Cation Traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
λ: 4 M in H2O
UV Absorption: λ: 260 nm Amax: 0.12; λ: 280 nm Amax: 0.08