Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is RNAse and DNAse-free. Tris is relatively non-hygroscopic; but, if needed, it can be dried at 100°C for up to 4 hours to remove any water.
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.
Grade: Ultra Pure
Purity: >99.9%
Key Applications: Electrophoresis
Product Type: Biochemicals
Biochemical Category: Buffers
Chemical Class: Biochemicals
Boiling Point: 219-220°C at 10 mmHg (Lit.)
Melting Point: 165-175°C
Vapor Pressure: Low at ambient temperatures
UV/Visible Absorbance: OD260 nm (5% aq soln) <0.1
Presentation: White Crystalline Powder
pH: 10-11.5 (5% aq soln)
pKa: 8.1 at 25°C (Lit.)
Loss on Drying: ≤1%
Solubility: Soluble in water (550 mg/mL), ethylene glycol (79.1 mg/mL), methanol (26 mg/mL), anhydrous ethanol (14.6 mg/mL), 95% ethanol (22.0 mg/mL), DMF (14 mg/mL), acetone (2 mg/mL), ethyl acetate (0.5 mg/mL), olive oil (0.4 mg/mL), and chloroform (0.05 mg/mL).
Storage & Handling: Store at Room Temperature, desiccate.