A Trident in-line guard cartridge holder can be used with almost any HPLC column by connecting it with a short piece of 1/16" tubing, appropriate nuts and ferrules, or finger-tight fittings. The system can be used with Restek columns, or with columns from other manufacturers. Holders are available for 10 mm guard cartridges. Purchase with or without a prefilter, which provides added protection against the particles that can shorten the lifetime of the guard cartridge.