Synonym: TSA, [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide
CAS Number: 58880-19-6
Empirical Formula (Hill Notation): C17H22N2O3
Molecular Weight: 302.37
MDL Number: MFCD03848392
PubChem Substance ID: 24900542
Preparation Note
Trichostatin A is soluble in methanol at 1.90 - 2.10 mg/ml and yields a clear, colorless to faint yellow/tan solution. It is also soluble in DMSO, DMF, acetonitrile, ethanol and lower alcohols. Trichostatin A is sparingly soluble in chloroform, ethyl acetate, acetone, and benzene. However, it is insoluble in water.
Application
Trichostatin A (TSA) has been used during the production of cloned mouse embryos1. TSA has also been used as a histone deacetylase (HDAC) inhibitor in LNCaP human prostate cancer cells2, as well as in MON and HeLa cells3.
Biochem/physiol Actions
Inhibits histone deacetylase at nanomolar concentrations; resultant histone hyperacetylation leads to chromatin relaxation and modulation of gene expression. May be involved in cell cycle progression of several cell types, inducing cell growth arrest at both G and G/M phases; may induce apoptosis. Enhances the efficacy of anticancer agents that target DNA.
Quality Level: PREMIUM
Biological Source: from Streptomyces sp.
Assay: ≥98% (HPLC)
Solubility: methanol: soluble1.90 - 2.10 mg/mL, clear, colorless to faint yellow or tan; DMF: soluble, DMSO: soluble, H2O: insoluble, acetone: slightly soluble, acetonitrile: soluble, benzene: slightly soluble, chloroform: slightly soluble, ethanol: soluble, ethyl acetate: slightly soluble, lower alcohols: soluble
Storage Temp.: −20°C
Gene Information: human ... (3065), (9759), (10013), (55869); mouse ... ENSMUSG00000061062(15181); rat ... (84582)