Transglutaminase from guinea pig liver, lyophilized powder, >=1.5 units/mg protein

Synonyms: Protein-Glutamine-gamma-Glutamyltransferase; Protein-glutamine:amine gamma-glutamyltransferase

CAS Number: 80146-85-6

MDL No.: MFCD00132446

Storage: -20C

Enzyme Commission (EC) Number: 2.3.2.13  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Application: This product from Sigma has been used to demonstrate that tissue transglutaminase (tTG) selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity. It has also been used to assess immune responses to A-gliadin peptides. Furthermore, it has been used to demonstrate that tTG selectively modifies gliadin peptides that are recognized by gut-derived T cells in celiac disease.

Application: Transglutaminase has been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington's disease and Alzheimer's disease.Transglutaminase has also been used in a study to develop a nonradioactive dot blot assay for transglutaminase activity.

Biochem/physiol Actions: Transglutaminase from guinea pig liver consists of a single polypeptide chain of 691 amino acid residues. It has six potential glycosylation sites (Asn-X-Ser or Asn-X-Thr), but it is not glycosylated. The molecular mass is approximately 76.6 kDa. It is calcium dependent and has several calcium binding sites. The enzyme is inhibited by iodoacetamide and N-ethylmaleimide in the presence of calcium. It catalyzes the incorporation of small molecular weight amines into γ-glutamine sites of proteins. In the absence of small molecular weight amines, it catalyzes the cross linking of proteins that results in the formation of γ-glutamyl-ε-lysine side chain peptides. Liver transglutaminase is a nonzymogenic enzyme.

Physical form: Lyophilized powder containing Tris and dithioerythritol

Unit Definition: One unit will catalyze the formation of 1.0 mumole of hydroxamate per min from Nalpha-Z-Gln-Gly and hydroxylamine at pH 6.0 at 37 C. (L-Glutamic acid gamma-monohydroxamate is the standard.)