Application
Uses sodium dodecyl sulfate for dissolving lipoproteins; offers excellent recovery of membrane proteins.
Features and Benefits
- Complete kit for protein assays
- Most widely cited protein assay
- Less protein to protein variation than other assays
- Contains reagents that can remove interfering substances
Principle
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.
Usage: sufficient for ~200 assays