Synonym: 9-[(dimethylamino)methyl]-10-hydroxy-(20S)-camptothecin hydrochloride hydrate, NSC-609669 hydrochloride hydrate, SKF-104864A hydrochloride hydrate, hycamptamine hydrochloride hydrate
CAS Number: 123948-87-8 (free base)
Empirical Formula (Hill Notation): C23H23N3O5 · xHCl · yH2dO
Molecular Weight: 421.45 (anhydrous free base basis)
MDL Number: MFCD00870670
Preparation Note
Topotecan hydrochloride hydrate is soluble in DMSO at a concentration that is greater than or equal to 20 mg/ml.
Application
Topotecan has been used as a positive control for the identification and analysis of HIF-1a and VEGF inhibitors in human glioma cells under hypoxic conditions1. It has also been used for in vitro apoptosis assays in PA317 cells2.
Biochem/physiol Actions
Topotecan is a topoisomerase I inhibitor and an apoptosis inducer. It is a potent antineoplastic agent.
Assay: =98% (HPLC and enzymatic)
Form: powder
Storage Condition: desiccated; protect from light
Color: yellow
Solubility: DMSO: =20 mg/mL
Storage Temp.: -20°C