Sharing 79% sequence identity with human Tumor Necrosis Factor-alpha (hTNF-a), mouse Tumor Necrosis Factor-alpha (mTNF-a) is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-a is further proteolytically processed to a soluble form [2]. The trimeric active TNF-a[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4].
Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-a) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.