Tumor necrosis factor-α (TNF-α) mediates many immune and inflammatory responses including activation and differentiation of monocytes and macrophages, expression of MHC class I and II, and expression of adhesion molecules on endothelial cells. Cayman’s TNF-α (mouse) ELISA Kit is an immunometric (i.e., sandwich) ELISA that permits TNF-α measurements within the range of 15.6-500 pg/ml, typically with an LLOQ of 15.6 pg/ml.