Recombinant human thrombospondin-1 is expressed in HEK293 cells as a glycoprotein with a calculated moleculaer mass of 127.5 kDa. This protein is manufactured in human cells using an all-human production system, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.
Synonyms: THBS; THBS1; TSP; TSP1; Thrombospondin-1
Purity: >=95% (SDS-PAGE)
Storage: -20C
Application: In rat C6 astroglioma cells, human thrombospondin 1 has been used in coating the cell culture plates to study the effects of integrins on CNTF (ciliary neurotrophic factor) expression. It has also been used in the preparation of secreted factor cocktail for analysis of the human mesenchymal stem cell secretome.Recommended for use as a cell culture substratum at 1-5 mug/cm2 or 0.1-25 mug/ml. Optimal concentration depends on cell type as well as the application or research objectives.