Thiazolyl Blue Tetrazolium Bromide, 98%

Synonym: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide

CAS Number: 298-93-1

Empirical Formula (Hill Notation): C₁₈H₁₆BrN₅S

Molecular Weight: 414.32

Beilstein Registry Number: 4081397

EC Number: 206-069-5

MDL Number: MFCD00011964

PubChem Substance ID: 24896603

Packaging
1, 5, 10 g in glass bottle; 100, 250, 500 mg in glass bottle

Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.^1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent⁶ and for the detection of NAD.⁷ ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.⁸ MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.⁹