Anti-TEM1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 46 and 81 kDa in Western Blots of specific cell lysates and tissues. Observed: 90 kD (post-modifications: 27 O-linked glycosylations) and 46kD. Positive controls used are human colon tissue, Caco2 Cells, MCF-7 cells, and SK-N-SH cells.