Tryptic Soy Broth (TSB) is for growth of nonfastidious and moderately fastidious microorganisms (e.g., Neisseria, Listeria, and Brucella). The combination of soy and casein peptones supply organic nitrogen in the form of amino acids and polypeptides, making the medium highly nutritious. Sodium chloride is added to maintain the osmotic equilibrium.
Contains 1.7% (17 g/L) tryptone, 0.3% (3 g/L) soytone, and 0.5% (5 g/L) sodium chloride, 0.25% glucose, and 0.25% dibasic potassium phosphate.