TCBS Agar (Selective) is recommended for selective isolation of Vibrio cholerae and other enteropathogenic Vibrio's. This medium was originally designed for the isolation of V. cholerae and V. parahaemolyticus, most Vibrios grow to healthy large colonies with many different colonial morphologies. TCBS Agar, Selective has an additional selective ingredient i.e. sodium cholate for improved selectivity. It is also recommended by APHA for the selective isolation of V.cholerae and V.parahaemolyticus. Enrichment in Alkaline Peptone Water (M618), followed by isolation on TCBS Agar is routinely used for isolation of V.cholerae.