Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile, Thermus aquaticus. The molecular weight of the recombinant protein is 94kD.. The Taq polymerase is able to amplify DNA up to 5kb with an elongation velocity of 0.9-1.2kb/min at 70-75°C. The error rate of this Taq polymerase is ~2.2x10
-5 nucleotide-1 cycle-1. Taq DNA polymerase catalyzes the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product
SOURCE A recombinant protein expressed in E. coli that carries the pol gene for Thermus aquaticus.
STORAGEBUFFER 20mM Tris.HCl (pH8.0), 100mM KCl, 3mM MgCl
2, 1mMDTT, 0.1% Nonidet® P-40, 0.1% Tween® 20, 0.2mg/ml BSA, 50% (v/v) glycerol.
10X PCR BUFFER 100mM Tris-HCL pH8.8, 500mM KCl, 16mM MgCl
2,1% TritonX-100
UNIT DEFINITION One unit (U) of Taq polymerase is defined as the amount of enzyme needed to catalyze the incorporation of 10 nanomoles of deoxyribonucleotides into acid-insoluble material in 30 minutes at 70°C using herring sperm DNA as a substrate.
Our Taq DNA Polymerase is offered in four convenient sizes and supplied with 10X PCR buffer (Mg
2+ plus). Taq Polymerase 2X Mastermix is a ready-to-use mixture of our high quality Taq DNA polymerase, deoxynucleotides and reaction buffer at a 2X concentration.
Features
- 5’→3’ polymerase activity
- No detectable 3’→5’ proofreading exonuclease activity
- Low 5’→3’ exonuclease activity
- Error rate ~2.2x10-5 nucleotide-1 cycle-1
- Available as a 2X Mastermix