T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. This enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters.
Application Features
Preparation of RNA vaccines
Radiolabeled RNA probes
Guide RNA for gene targeting
mRNA for in vitro translation and micro injection
RNA amplification
Product Source
Isolated from E. coli carrying a plasmid which contains the T7 RNA Polymerase gene.
1X T7 RNA Polymerase Reaction Buffer
40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine (pH 7.9 @ 25°C)
Storage Buffer
50 mM Tris-HCl, 100 mM NaCl, 20 mM β-ME, 1 mM EDTA, 50% Glycerol, 0.1% (w/v) Triton® X-100, pH 7.9 @ 25°C
Reaction Conditions
1X T7 RNA Pol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C.