T4 recombination mediator protein, UvsY plays important roles in homologous recombination (HR). During HR, the UvsX recombinase competes with the prebound single-stranded binding protein gp32 for DNA-binding sites and UvsY stimulates this filament nucleation event. UvsY stimulates the DNA-dependent ATPase activity of UvsX, lowers the critical concentration of UvsX that is required for activity, and promotes strand exchange. UvsY efficiently promotes the UvsX-catalyzed strand invasion reaction by recruiting to ssDNA–gp32 complexes, promotes the release of gp32, and favors the binding of UvsX.
Product Source
E. coli cells expressing T4 UvsY gene
Product Includes
- T4 UvsY protein
- 10X UvsY reaction buffer
10X UvsY reaction buffer composition
200 mM Tris-acetate
1M Potassium acetate
100 mM Magnesium acetate
10 mM DTT
pH 7.8 @ 25ºC
Storage Buffer
50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25ºC
Storage Temperature: -20ºC
Quality Control assays
UvsY is free from detectable nuclease contamination.