T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesized strand displacing the old strand. The newly synthesized duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA
Product Source
E. coli cells expressing recombinant T4 UvsX gene.
Product Includes
- T4 UvsX DNA Recombinase
- 10X UvsX DNA Recombinase reaction buffer
10X UvsX DNA Recombinase reaction buffer composition
200 mM Tris-acetate pH 7.8
1M Potassium acetate
100 mM Magnesium acetate
10 mM DTT
Storage Buffer
50 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.5 @ 25ºC
Storage Temperature
- 20ºC
Quality Control assays
UvsX DNA recombinase is free from detectable nuclease contamination.