T4 Polynucleotide Kinase (PNK) catalyzes the transfer of the γ-phosphate from ATP to the 5’-OH group of single- and double-stranded DNAs and RNAs, oligonucleotides or nucleoside 3’-monophosphates. In the presence of ADP, T4 PNK exhibits 5’-phosphatase activity and catalyzes the exchange of phosphate group between 5’-P-oligonucleotides/polynucleotides and ATP. The enzyme is also a 3’-phosphatase.
Product Includes:
T4 Polynucleotide Kinase (PNK)
10X T4 PNK Reaction Buffer
Applications
Labeling 5′-termini of nucleic acids (3, 4)
5′-phosphorylation of oligonucleotides, PCR products, other DNA or RNA prior to ligation.
Phosphorylation of PCR primers.
Detection of DNA modification by the [32P]-postlabeling assay (5, 6).
Removal of 3′-phosphate groups (2).