T4 GP32 is a single-stranded DNA binding protein with key role in DNA replication, recombination and repair. T4 GP32 binds cooperatively to single stranded DNA and stimulates cell-free DNA synthesis directed by the T4-encoded DNA polymerase. It improves the efficiency of reverse transcriptase during RT-PCR as well as increases the yield of PCR products.
Product Source
E. coli cells expressing T4 gp32 gene.
Product Includes
- T4 GP32 protein
- 10X GP32 reaction buffer
10X GP 32 reaction buffer composition
200 mM Tris-acetate
1M Potassium acetate
100 mM Magnesium acetate
10 mM DTT
pH 7.8 @ 25ºC
Storage Temperature: -20ºC
Heat Inactivation: 65°C for 20 min
Quality Control assays
T4 GP32 protein is free from detectable nuclease contamination.