T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction with the presence of template and primer. It has both DNA-dependent DNA polymerase activity and 3´→5´ exonuclease activity which make the enzyme useful for several molecular biology applications such as:
- 3´-overhang removal to form blunt ends.
- 5´-overhang fill-in to form blunt ends.
- Probe labeling using replacement synthesis.
- DNA library preparation for Next-generation sequencing.
- Ligation-independent cloning of PCR products.
- Second strand synthesis in site-directed mutagenesis.
Source
E. coli cells with a cloned gene of bacteriophage T4 DNA Polymerase
Product includes
- T4 DNA Polymerase
- 10X T4 DNA Polymerase buffer
10X T4 DNA Polymerase reaction buffer composition
500 mM Tris-HCl, 100 mM MgCl2, 50 mM dithiothreitol, pH 7.5 @ 25°C.
Storage Buffer
50 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.5 @ 25ºC.
Unit Definition:
One unit of T4 DNA Polymerase converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard assay conditions.
Molecular Weight
Theoretical: 104 kDa
Storage temperature
- 20°C.