Intact Genomics T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzymes joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Intact Genomics T4 DNA Ligase displays significantly higher ligation efficiency than the nearest competitor.
Product Includes:
T4 DNA Ligase
10x T4 DNA Ligase Reaction Buffer
• 3-5X higher ligation efficiency than the nearest competitor.
•Extremely high purity.
• Useful for Next-gen library preparation, Cloning of PCR products, Self-circularization of linear DNA and more.
• Quality control is performed following the production of each new lot of product to ensure that it meets the quality standards and specifications designated for the product.
• The physical purity of this enzyme is ≥99% as assessed by SDS-PAGE with Coomassie® blue staining.