The SygRNA(R) synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides Cas9 protein to crRNA-specified sites in the genome. SygRNA(R) synthetic tracrRNA and crRNAs are optimized for use with Cas9 expressing of cell lines, Cas9 protein, or Cas9 mRNA and compatible with a variety of delivery methods such as microinjection, lipofection, and electroporation.
Storage: -20C
Application: Functional Genomics/Transgenic Applications
•Creation of gene knockouts
•Creation of knock-in animals or cell lines with promoters, fusion tags or reporters integrated into endogenous genes
Legal Information: SygRNA is a registered trademark of Sigma-Aldrich Co. LLC