Superoxide dismutase (SOD) is part of a family of metal ion-containing enzymes (SODs) that are found in most organisms, from bacteria to mammals. SODs catalyze the dismutation of superoxide (generated by aerobic respiration) to molecular oxygen and peroxide. Superoxide dismutase form the front line of defence against reactive oxygen species (ROS)-mediated injury and harbor anti-inflammatory activities. The Superoxide Dismutase (SOD) Activity Assay Kit provides a simple and sensitive procedure for measuring SOD enzymatic activity in various sample types. SOD activity is determined by measuring the decrease in superoxide anions (generated by the enzyme xanthine oxidase [XO]). Since superoxide anions interact with the provided WST dye, yielding color at 450 nm, the decrease in the color signal is proportional to SOD inhibition activity. The kit allows the determination of SOD activity in percent inhibition, or in units of activity using the provided SOD Enzyme as a standard. The provided SOD Enzyme can also be used to screen for SOD inhibitors. The kit's lowest limit of detection is 0.3 SOD units/mL. This kit can be used to measure SOD activity in biological samples such as serum, plasma and erythrocytes, tissue homogenates and cell lysates. Components This kit contains sufficient reagents for 500 colorimetric tests. Our economical Superoxide Dismutase (SOD) activity assay kit also saves your time and efforts:
•Highly sensitive, simple and high-throughput assay for measuring SOD activity in a variety of biological samples (serum, plasma,erythrocytes, tissue homogenates and cell lysates)
•Allows measurement of SOD activity both in percentage of inhibition and also by measuring the activity in enzymatic units using a calibration curve curve.
•Enables inhibitor screen using our SOD enzymes which is provided as a standard.
•User friendly: No need to weigh or calculate needed amounts of reagents on your own
•Supportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
•Quick instruction bench card - to assure your experimental success
•More experiments in one kit - contains sufficient reagents for 500 colorimetric tests.
•Limit of detection: 0.3 SOD units/mL
•Detection time: only 30 min
•Detection can be done at room temperature.
Synonyms: CU/ZN-SOD; SOD; SOD1; Superoxide Dismutase; Superoxide Dismutase 1 bovine; Superoxide: superoxide oxidoreductase
Storage: -20C