Guard columns are placed before the analytical column to protect and prevent harmful materials entering into the latter. It also helps as a backup to the in-line filter and also checks for leak through filter. The most suitable are short cartridge which is inserted into the cartridge holder.
Application: SUPELCOSIL(TM) LC-18 Supelguard(TM) Cartridge was used as guard column for Supelcosil(TM) LC-18 HPLC column to analyze glucose tetrasaccharides in blood and urine samples using HPLC-electrospray ionization mass spectrometry (HPLC-ESI-MS). It was also suitable for analysing polar compounds in non-polar matrices using gas chromatography separation.
Legal Information: Supelguard is a trademark of Sigma-Aldrich Co. LLC