The Sulfhydryl Coupling Resin is designed for the simple and efficient coupling of peptides and proteins to a solid 6% agarose support through free sulfhydryl groups (-SH).The iodoacetyl groups of the Sulfhydryl Coupling Resin specifically react with free sulfhydryls to form covalent, permanent thioether bonds (see figure).The long spacer arm reduces steric hindrance and ensures greater binding of proteins and antibodies during affinity purification.
Features
- Simple & efficient coupling of peptides & proteins
- Stable coupling of proteins & peptides to form covalent thioether bonds
- Long spacer arm for reduced steric hindrance &greater protein & antibody binding
- Couples 1-2mg peptide & 2-20mg protein/ml resin
Applications
- Immobilization & coupling of free sulfhydryls of peptides, proteins & ligand s
- Antibody purification
- Affinity Chromatography