Synonym: α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar
CAS Number: 57-50-1
Empirical Formula (Hill Notation): C12H22O11
Molecular Weight: 342.30
Beilstein Registry Number: 90825
EC Number: 200-334-9
MDL Number: MFCD00006626
PubChem Substance ID: 24888177
Other Notes
Isopycnic centrifugation in a discontinuous sucrose density gradient e.g. in the isolation of mRNA from membrane-bound polysomes.
Application
Sucrose is a widely used nutitional disaccharide of glucose and fructose. Sucrose is used in molecular biology for the preparation of density gradients.
Grade: for molecular biology
Product Line: BioUltra
Assay: ≥99.5% (HPLC)
Optical Activity: [α]20/D +66.5±1°, c = 26% in H2O
Impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
Ign. Residue: ≤0.01% (as SO4)
Loss: ≤0.1% loss on drying, 20°C (HV)
pH: 5.0-7.0 (25 °C, 1 M in H2O)
MP: 185-187 °C(lit.)
Solubility: H2O: soluble1 M at 20 °C, clear, colorless
Anion Traces: chloride (Cl-): ≤50 mg/kg; sulfate (SO42-): ≤50 mg/kg
Cation Traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
λ : 1 M in H2O
UV Absorption: λ: 260 nm Amax: 0.11; λ: 280 nm Amax: 0.08