Product contains enough liquid concentrate to make 1L of 1X solution. To use this product, dilute it 4 fold with DI water and add 0.6% beta ME. Wash membrane in this buffer and incubate at 65°C for 30 minutes under constant agitation. Wash the membrane 5X for 5 min in PBS-Tween20 or TBS-Tween20.
Technical Information:
- Contains: 250mM Tris, 8% SDS
- pH@ 25°C: 6.8
- Add: 0.6% β-Mercatpoethanol
- Hazard: Irritant