Spectinomycin Dihydrochloride Pentahydrate, Powder, BioReagent, Suitable for cell culture

CAS Number: 22189-32-8

Empirical Formula (Hill Notation): C₁₄H₂₄N₂O₇ · 2HCl · 5H₂O

Molecular Weight : 495.35

Application
Recommended for use in cell culture applications at 7.5-20 mg/L and as a selection agent for transformed plant cells that contain the selectable marker gene Spcr.

Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis (elongation) by interfering with peptidyl tRNA translocation.
Antimicrobial spectrum: Gram-negative and Gram-positive bacteria (Gonnococcus only).
Mode of Resistance: Mutation in rpsE (the gene for ribosomal protein S5) prevents binding of spectinomycin.

Reconstitution
Prepare stock solutions should be prepared (water 10 mg/ml) and filter sterilized. Stock solutions can be stored at 2-8 °C for several weeks or at -20 °C for extended periods.