SM(PEG)n NHS- and maleimide-activated PEG compounds for crosslinking between primary amines (NH2) and sulfhydryl (SH) groups in proteins and other molecules. A complete set of compounds are offered, each having the same heterobifunctional structure (NHS-PEGn-Maleimide) but differing in the number of discrete ethylene glycol units ( n = 2, 4, 6, 8, 12 or 24). The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The maleimide group reacts with reduced sulfhydryls at pH 6.5-7.5 to form stable thioether bonds
Molecular Formula: C62H111N3O31
Linear Structural Formula: C62H111N3O31
Storage: -20C
Application: SM(PEG)24 can be used as a crosslinker in:
•The study of chemical cross-linking of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and RNAses using the high-mass MALDI-MS technique.
•Preparation of protein G-liposomal nanovesicles applicable as sensors in the detection of gentamycin in the milk sample.
•Conjugation of catalase to streptavidin, which is helpful in the detection of analytes at very low concentrations using ELISA technique.