Storage: -196C
UNSPSC Code: 12352200
Application: For complete product information, please see ECACC
Application: Recommended culture medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Cell Line Origin: Oesophagus adenocarcinoma, well-differentiated
Culture Medium: RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Cell population doubling time 39 hours.
Other Notes: Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
RIDADR: NONH for all modes of transport