Purity: ≥95% (SDS-PAGE)
General description: HumanKine VEGF165 is expressed in human HEK 293 cells as a glycosylated homodimer with an apparent molecular mass of 45 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
Analysis Note: The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).
Legal Information: HumanKine is a registered trademark of HumanZyme Inc.
Biochem/physiol Actions: VEGF165 belongs to the PDGF/VEGF growth factor family. Many cell types secrete VEGF, and it is a potent angiogenic factor and mitogen that stimulates proliferation, migration, and formation of endothelial cells. VEGF stimulates permeabilization of blood vessels and is present in some tumors of the nervous system. VEGF is induced by hypoxia, oncogene mutations, and cytokines such as IL-1, IL-8, TNF-α.
Empirical Formula (Hill Notation): C6H10