Synonyms: TRIS HCl; TRIS hydrochloride; Tris(hydroxymethyl)aminomethane hydrochloride
Formula: NH2C(CH2OH)3 · HCl
Formula Weight: 157.60
CAS No.: 1185-53-1
Purity: ≥99.0% (titration)
Application: The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Other Notes: Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Legal Information: Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Abs.: A0.5M/260, H2O ≤0.015
Anion Traces: sulfate (SO42-): ≤0.005%
Assay: ≥99.0% (titration)
Cation Traces: As: ≤0.0001%
Ign. Residue (900°C): ≤0.2% (as SO4)
Impurities: Insoluble matter, passes filter test
Loss: ≤0.2% loss on drying, 110°C
pH: 3.5-5.0 (0.5 M in H2O)
pKa (25°C): 8.1
Product Line: BioXtra
Quality Level: ELITE
Solubility: H2O: soluble0.5 M, clear, colorless
Useful pH Range: 7.0 - 9.0