Synonyms: 2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol
Formula: NH2C(CH2OH)3
Formula Weight: 121.14
CAS No.: 77-86-1
Purity: ≥99.9% (titration)
Analysis Note: Not pyrogen tested. Certificates of analysis are available with lot specific information.
Other Notes: The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information: Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Packaging: Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.
Assay: ≥99.9% (titration)
BP: 219-220°C/10 mmHg(lit.)
Color: yellow tan
Description: aminopeptidase substrate
Form: crystalline
MP: 167-172°C(lit.)
pKa (25°C): 8.1
Quality Level: ELITE
Useful pH Range: 7 - 9