Synonyms: 2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol
Formula: NH2C(CH2OH)3
Formula Weight: 121.14
CAS No.: 77-86-1
Purity: ≥99.0% (T)
Other Notes: The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information: Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Anion Traces: sulfate (SO42-): ≤50 mg/kg
Assay: ≥99.0% (T)
BP: 219-220°C/10 mmHg(lit.)
Cation Traces: Cr: ≤5 mg/kg
Description: aminopeptidase substrate
Loss: ≤1% loss on drying, 110 °C
MP: 168-172°C
pKa (25°C): 8.1
Solubility: H2O: soluble1 M at 20°C, clear, colorless
Useful pH Range: 7 - 9