SIGMA Tris Acetate-EDTA buffer, DNase and RNase, none detected, BioReagent, suitable for electrophoresis, 10x concentrate

Synonyms: TAE buffer

Application: Ready for use in gel electrophoresis after dilution to working concentrations.

Preparation Note: Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Reconstitution: Produces a 10× concentrate (0.4 M Tris-acetate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

Packaging: Packaged in pouches