Taq DNA Polymerase from Thermus aquaticus, with 10x PCR reaction buffer without MgCl2

CAS Number: 9012-90-2

MDL No.: MFCD00166026

Storage: -20C

Enzyme Commission (EC) Number: 2.7.7.7  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Features and Benefits: . MgCl2 provided in a separate tube to allow MgCl2 optimization

General description: Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80C) has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA.

Application: Taq DNA Polymerase from Thermus aquaticus has been used in the process of DNA extraction (during gene amplification and sequencing). It has been used in genotyping. It has also been used in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8).

Application: Taq DNA Polymerase from Thermus aquaticus is a thermostable DNA polymerase that is used for the DNA polymerase chain reaction (PCR) in order to amplify DNA sequences.

Biochem/physiol Actions: Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5' to 3' polymerase and exonuclease activities.

Unit Definition: One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 C.

Other Notes: Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5'->3' polymerase and exonuclease activity.