Components: Assay Buffer 20 mL; SIRT1 Substrate (Fluorometric) 100 μL; Standard (non-acetylated) 20 mM 100 μL; Developer Solution 1.5 mL; Nicotinamide Solution (inhibitor) 100 μL; SIRT1 human, recombinant, expressed in E. coli, N-terminal histidine tagged, ≥90% (SDS-PAGE), buffered aqueous glycerol solution 150 μg; NAD+ Solution 1 mL; Resveratrol Solution (activator) 100 μL