Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.
MDL Number: MFCD00217820
Storage: 2-8C
Application: RPMI-1640 Medium is used for the following applications:
•used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)
•used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
•used for cell isolation and culture
•used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay
•used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
•used for the preparation of antifungal agents
Quantity: Formulated to contain 10.4 grams of powder per liter of medium.
Reconstitution: Supplement with 2.0 g/L sodium bicarbonate.
Empirical Formula (Hill Notation): C10H10O4