REDAccuTaq(R) LA DNA Polymerase, High fidelity Taq with inert dye, 10X buffer included

MDL No.: MFCD01092095

Storage: -20C

UNSPSC Code: 12352200

Features and Benefits: . Increased fidelity, up to 6.5x that of Taq DNA polymerase. Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA. Visual confirmation that enzyme has been added, and proper mixing has occurred. No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis

General description: REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.

Application: Produce long DNA amplicons for:. Mutation analysis. Cloning genes. DNA sequencing. cDNA library generation

Unit Definition: One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 C.

Other Notes: Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at -20 C.

Other Notes: REDAccuTaq(R) LA DNA polymerase is a blend of Sigma's high quality Taq DNA polymerase, a small amount of an additional polymerase that exhibits 3'->5' exonuclease or proofreading activity, and our inert red dye. By blending the Taq with the right amount of this proofreading enzyme, misincorporation errors are corrected, producing PCR amplicons that are longer and more accurate.