Purity: ≥85% (HPLC)
Application: R3 IGF-I is a recombinant analog of insulin-like growth factor containing the complete human IGF-I amino acid sequence with substitution of Arg for Glu3. It was developed as an inexpensive, high quality potent analog of IGF for use as a growth factor supplement for serum-free or low serum cell culture.
Recombinantly produced in E. coli using a patented expression system, the peptide is harvested from cells in inclusion bodies, which are dissolved and desalted. The active molecule is properly folded under oxidizing conditions and then purified by several liquid chromatography steps.
Preparation Note: Prepared from 0.1 M acetic acid
Other Notes: Recombinant analog of insulin-like growth factor-I with the substitution of Arg for Glu at position 3.
Storage: 2-8°C
Biochem/physiol Actions: Product stimulates protein synthesis in L6 myoblasts.
Empirical Formula (Hill Notation): Br3OP