Pyroglutamate Aminopeptidase from Pyrococcus furiosus, recombinant, expressed in E. coli, ~90% (SDS-PAGE), >=5.0 units/mg protein

Synonyms: L-Pyrrolidone carboxyl peptidase

MDL No.: MFCD00132139

Purity: ~90% (SDS-PAGE)

Storage: -20C

Enzyme Commission (EC) Number: 3.4.19.3  ( BRENDA | IUBMB )

EC Number: 232-981-8

UNSPSC Code: 12352204

Application: The enzyme from Sigma has been used for the removal of pyroglutamate (pGlu) N-terminal blocking group, under reduced conditions, prior to N-terminal sequencing of purified cassiicolin.

Application: Thermostable aminopeptidase that liberates N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation.

Application: Pyroglutamate Aminopeptidase, from Pyrococcus furiosus is a recombinant, thermostable aminopeptidase that is expressed in Escherichia coli. It is used to cleave pyroglutamic acid which allows analysis of N-terminal sequences of peptides.

Biochem/physiol Actions: This enzyme is specific for N-terminal pyroglutamic acids. It cleaves the N-terminal pyroglutamic acid from proteins and peptides prior to Edman degradation. The optimal temperature range is 95 to 100 C and the optimal pH range is 6.0 to 9.0.

Physical form: Lyophilized powder containing sodium phosphate

Unit Definition: One unit will hydrolyze 1 mumol of pyroglutamate p-nitroanilide per minute at pH 7.0 at 37 C.

Preparation Note: Reconstitute the vial of enzyme with 50 mul of 50 mM sodium phosphate, pH 7.0, with 10 mM DTT and 1 mM EDTA. The reconstituted solution should be stored at -20 C.