Protein Kinase G Ibeta human, >=95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

Synonyms: cyclic-Guanosine Monophosphate-dependent Protein Kinase 1beta human

MDL No.: MFCD03458230

Purity: >=95% (SDS-PAGE)

Activity: activity: >=1.5 units/mg protein (20-fold stimulation by cGMP (5 muM))

Storage: -20C

UNSPSC Code: 12352204

Application: Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.

Biochem/physiol Actions: Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.

Physical form: Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM beta-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.

Unit Definition: One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.